Publications

2026

Farrukh HSUB, Farrukh A, Hambardzumyan S, Steudter T, Pearson S, Campo AD. Thiol-Methylsulfone Crosslinked Hydrogels for Cell Encapsulation: Molecular Scale Modulation of Physiochemical Properties. Macromol Biosci. 2026 Feb;26(2):e00627. doi: 10.1002/mabi.202500627. PMID: 41733188; PMCID: PMC12931027.

Desai K, Garcia-Sanchez L, Amini M, Teruel-Enrico LL, Siegrist S, Del Campo A, Trujillo S. Evaluating Cytocompatibility of Corynebacterium glutamicum-poly Vinyl Alcohol Living Biomaterials for Ocular Use. Adv Healthc Mater. 2026 Mar;15(9):e03831. doi: 10.1002/adhm.202503831. Epub 2025 Nov 29. PMID: 41316961; PMCID: PMC12973338.

Muhovic B, Bartolome MP, Enrico LLT, Dupont L, Jonas AM, Glinel K, Del Campo A, Wittmann C. Engineering Corynebacterium glutamicum as a multifunctional biofactory for living therapeutic materials and controlled ectoine delivery. Biomater Adv. 2026 Mar 30;185:214847. doi: 10.1016/j.bioadv.2026.214847. Epub ahead of print. PMID: 41950672.

Hambardzumyan S, Kasper JY, Del Campo A. Quantification of collagen matrix deposition in 2D cell cultures: a comparative study of existing assays. Biomater Adv. 2026 Jan;178:214436. doi: 10.1016/j.bioadv.2025.214436. Epub 2025 Jul 29. PMID: 40782599.

2025

Reinhold S, Herr C, Yao Y, Pourrostami M, Ritzmann F, Lehr T, Selzer D, Kohl Y, Yildiz D, Slevogt H, Beisswenger C, Bals R. Modeling of lung-liver interaction during infection in a human fluidic organ-on-a-chip. Sci Rep. 2025 Oct 9;15(1):35241. doi: 10.1038/s41598-025-22682-z. PMID: 41068301; PMCID: PMC12511430.

Chen G, Wiegand C, Willett A, Herr C, Müller R, Bals R, Kalinina OV. Comparative metagenomic analysis on COPD and health control samples reveals taxonomic and functional motifs. Front Microbiol. 2025 Nov 26;16:1636322. doi: 10.3389/fmicb.2025.1636322. PMID: 41383738; PMCID: PMC12689518.

Oberhäuser P, Myronovskyi M, Stierhof M, Gromyko O, Luzhetskyy A. Identification and heterologous expression of an NRPS biosynthetic gene cluster responsible for the production of the pyrazinones Ichizinone A, B and C. Microb Cell Fact. 2025 Jun 7;24(1):131. doi: 10.1186/s12934-025-02753-6. PMID: 40481494; PMCID: PMC12144821

Mekontso JA, Farrukh U, Trujillo S, Del Campo A. A practical workflow for cytocompatibility assessment of living therapeutic materials. Biomater Adv. 2025 Apr;169:214182. doi: 10.1016/j.bioadv.2025.214182. Epub 2025 Jan 10. PMID: 39826261.

Adaptations of Gram-Negative and Gram-Positive Probiotic Bacteria in Engineered Living Materials Varun Sai Tadimarri, Tanya Amit Tyagi, Cao Nguyen Duong, Sari Rasheed, Rolf Müller, and Shrikrishnan Sankaran ACS Biomaterials Science & Engineering 202511 (6), 3773-3784 DOI: 10.1021/acsbiomaterials.5c00325

Desai K, Mekontso J, Deshpande K, Trujillo S. Preclinical Assessment of Living Therapeutic Materials: State-of-Art and Challenges. ACS Biomater Sci Eng. 2025 Apr 15. doi: 10.1021/acsbiomaterials.5c00247. Epub ahead of print. PMID: 40230223.

Khazem A, Schmachtenberg R, Weiand A, Sankaran S, Weber W. Engineered microbial living matter for diagnostics, prevention, and therapy. Curr Opin Biotechnol. 2025 Apr;92:103269. doi: 10.1016/j.copbio.2025.103269. Epub 2025 Feb 6. PMID: 39919667.

Kafrashian Z, Brück S, Rogin P, Khamdan M, Farrukh HSUB, Pearson S, Del Campo A. Segmented, Side-Emitting Hydrogel Optical Fibers for Multimaterial Extrusion Printing. Adv Mater. 2025 Jan;37(4):e2309166. doi: 10.1002/adma.202309166. Epub 2024 Dec 4. PMID: 39632473; PMCID: PMC11775871.

Tadimarri VS, Blanch-Asensio M, Deshpande K, Baumann J, Baumann C, Müller R, Trujillo S, Sankaran S. PEARL: Protein Eluting Alginate with Recombinant Lactobacilli. Small. 2025 Jan 28:e2408316. doi: 10.1002/smll.202408316. Epub ahead of print. PMID: 39871788.

Tadimarri VS, Sankaran S. Optogenetic Engineered Living Materials. Methods Mol Biol. 2025;2840:101-114. doi: 10.1007/978-1-0716-4047-0_8. PMID: 39724347.

2024

Desai K, Sankaran S, Del Campo A, Trujillo S. A screening setup to streamline in vitro engineered living material cultures with the host. Mater Today Bio. 2024 Dec 30;30:101437. doi: 10.1016/j.mtbio.2024.101437. PMID: 39850240; PMCID: PMC11755081.

Dey S, Seyfert CE, Fink-Straube C, Kany AM, Müller R, Sankaran S. Thermo-amplifier circuit in probiotic E. coli for stringently temperature-controlled release of a novel antibiotic. J Biol Eng. 2024 Nov 12;18(1):66. doi: 10.1186/s13036-024-00463-y. PMID: 39533331; PMCID: PMC11559228 2

Schmartz GP, Rehner J, Gund MP, Keller V, Molano LG, Rupf S, Hannig M, Berger T, Flockerzi E, Seitz B, Fleser S, Schmitt-Grohé S, Kalefack S, Zemlin M, Kunz M, Götzinger F, Gevaerd C, Vogt T, Reichrath J, Diehl L, Hecksteden A, Meyer T, Herr C, Gurevich A, Krug D, Hegemann J, Bozhueyuek K, Gulder TAM, Fu C, Beemelmanns C, Schattenberg JM, Kalinina OV, Becker A, Unger M, Ludwig N, Seibert M, Stein ML, Hanna NL, Martin MC, Mahfoud F, Krawczyk M, Becker SL, Müller R, Bals R, Keller A. Decoding the diagnostic and therapeutic potential of microbiota using pan-body pan-disease microbiomics. Nat Commun. 2024 Sep 26;15(1):8261. doi: 10.1038/s41467-024-52598-7. PMID: 39327438; PMCID: PMC11427559.

Puertas-Bartolomé M, Gutiérrez-Urrutia I, Teruel-Enrico LL, Duong CN, Desai K, Trujillo S, Wittmann C, Del Campo A. Self-Lubricating, Living Contact Lenses. Adv Mater. 2024 Jul;36(27):e2313848. doi: 10.1002/adma.202313848. Epub 2024 Apr 21. PMID: 38583064.

Dey S, Sankaran S. Engineered bacterial therapeutics with material solutions. Trends Biotechnol. 2024 Dec;42(12):1663-1676. doi: 10.1016/j.tibtech.2024.06.011. Epub 2024 Jul 18. PMID: 39030122.

Präve L, Seyfert CE, Bozhüyük KAJ, Racine E, Müller R, Bode HB. Investigation of the Odilorhabdin Biosynthetic Gene Cluster Using NRPS Engineering. Angew Chem Int Ed Engl. 2024 Aug 12;63(33):e202406389. doi: 10.1002/anie.202406389. Epub 2024 Jul 10. PMID: 38801753.

Beganovic S, Wittmann C. Medical properties, market potential, and microbial production of golden polyketide curcumin for food, biomedical, and cosmetic applications. Curr Opin Biotechnol. 2024 Jun;87:103112. doi: 10.1016/j.copbio.2024.103112. Epub 2024 Mar 22. PMID: 38518404.

Dey S, Sankaran S. Sustainable protein regeneration in encapsulated materials. Cell Syst. 2024 Mar 20;15(3):211-212. doi: 10.1016/j.cels.2024.02.004. PMID: 38513614.

Vella L, Sternjakob A, Lohse S, Fingerle A, Sperling T, Wickenhauser C, Stöckle M, Vogt T, Roemer K, Ołdak M, Smola S. The cutaneous beta human papillomavirus type 8 E6 protein induces CCL2 through the CEBPα/miR-203/p63 pathway to support an inflammatory microenvironment in epidermodysplasia verruciformis skin lesions. Front Cell Infect Microbiol. 2024 Mar 6;14:1336492. doi: 10.3389/fcimb.2024.1336492. PMID: 38510961; PMCID: PMC10953690.

Blanch-Asensio M, Tadimarri VS, Wilk A, Sankaran S. Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering. Microb Cell Fact. 2024 Feb 7;23(1):42. doi: 10.1186/s12934-024-02302-7. PMID: 38326819; PMCID: PMC10848424.

Bhusari S, Hoffmann M, Herbeck-Engel P, Sankaran S, Wilhelm M, Del Campo A. Rheological behavior of Pluronic/Pluronic diacrylate hydrogels used for bacteria encapsulation in engineered living materials. Soft Matter. 2024 Feb 7;20(6):1320-1332. doi: 10.1039/d3sm01119d. PMID: 38241053.

Brand M, Ritzmann F, Kattler K, Milasius D, Yao Y, Herr C, Kirsch SH, Müller R, Yildiz D, Bals R, Beisswenger C. Biochemical and transcriptomic evaluation of a 3D lung organoid platform for pre-clinical testing of active substances targeting senescence. Respir Res. 2024 Jan 3;25(1):3. doi: 10.1186/s12931-023-02636-7. PMID: 38172839; PMCID: PMC10765931

Blanch-Asensio M, Dey S, Tadimarri VS, Sankaran S. Expanding the genetic programmability of Lactiplantibacillus plantarum. Microb Biotechnol. 2024 Jan;17(1):e14335. doi: 10.1111/1751-7915.14335. Epub 2023 Aug 28. PMID: 37638848; PMCID: PMC10832526.

Weiland F, Barton N, Kohlstedt M, Becker J, Wittmann C. Systems metabolic engineering upgrades Corynebacterium glutamicum to high-efficiency cis, cis-muconic acid production from lignin-based aromatics. Metab Eng. 2023 Jan;75:153-169. doi: 10.1016/j.ymben.2022.12.005. Epub 2022 Dec 20. PMID: 36563956

2023

Seyfert CE, Müller AV, Walsh DJ, Birkelbach J, Kany AM, Porten C, Yuan B, Krug D, Herrmann J, Marlovits TC, Hirsch AKH, Müller R. New Genetically Engineered Derivatives of Antibacterial Darobactins Underpin Their Potential for Antibiotic Development. J Med Chem. 2023 Dec 14;66(23):16330-16341. doi: 10.1021/acs.jmedchem.3c01660. Epub 2023 Nov 21. PMID: 38093695; PMCID: PMC10726357.

Riedel F, Bartolomé MP, Enrico LLT, Fink-Straube C, Duong CN, Gherlone F, Huang Y, Valiante V, Del Campo A, Sankaran S. Engineered living materials for the conversion of a low-cost food-grade precursor to a high-value flavonoid. Front Bioeng Biotechnol. 2023 Nov 28;11:1278062. doi: 10.3389/fbioe.2023.1278062. PMID: 38090710; PMCID: PMC10715425.

Eckert N, Rebets Y, Horbal L, Zapp J, Herrmann J, Busche T, Müller R, Kalinowski J, Luzhetskyy A. Discovery and overproduction of novel highly bioactive pamamycins through transcriptional engineering of the biosynthetic gene cluster. Microb Cell Fact. 2023 Nov 14;22(1):233. doi: 10.1186/s12934-023-02231-x. PMID: 37964282; PMCID: PMC10644645.

Trujillo S, Kasper J, de Miguel-Jiménez A, Abt B, Bauer A, Mekontso J, Pearson S, Del Campo A. Cytocompatibility Evaluation of PEG-Methylsulfone Hydrogels. ACS Omega. 2023 Aug 23;8(35):32043-32052. doi: 10.1021/acsomega.3c03952. PMID: 37692225; PMCID: PMC10483518.

Yanamandra AK, Bhusari S, Del Campo A, Sankaran S, Qu B. In vitro evaluation of immune responses to bacterial hydrogels for the development of living therapeutic materials. Biomater Adv. 2023 Oct;153:213554. doi: 10.1016/j.bioadv.2023.213554. Epub 2023 Jul 13. PMID: 37480604.

Knebel M, Körner S, Kühn JP, Wemmert S, Brust L, Smola S, Wagner M, Bohle RM, Morris LGT, Pandey A, Schick B, Linxweiler M. Prognostic impact of intra- and peritumoral immune cell subpopulations in head and neck squamous cell carcinomas – comprehensive analysis of the TCGA-HNSC cohort and immunohistochemical validation on 101 patients. Front Immunol. 2023 Jun 13;14:1172768. doi: 10.3389/fimmu.2023.1172768. PMID: 37383237; PMCID: PMC10294051

Dey S, Blanch-Asensio M, Balaji Kuttae S, Sankaran S. Novel genetic modules encoding high-level antibiotic-free protein expression in probiotic lactobacilli. Microb Biotechnol. 2023 Jun;16(6):1264-1276. doi: 10.1111/1751-7915.14228. Epub 2023 Feb 1. PMID: 36722614; PMCID: PMC10221531.

P. Dhakane, V. S. Tadimarri, S. Sankaran Light-Regulated Pro-Angiogenic Engineered Living Materials Advanced Functional Materials 2023, 2212695

Basaran S, Dey S, Bhusari S, Sankaran S, Kraus T. Plasmonic stimulation of gold nanorods for the photothermal control of engineered living materials. Biomater Adv. 2023 Apr;147:213332. doi: 10.1016/j.bioadv.2023.213332. Epub 2023 Feb 14. PMID: 36801796.

Srikakulam SK, Keller S, Dabbaghie F, Bals R, Kalinina OV. MetaProFi: an ultrafast chunked Bloom filter for storing and querying protein and nucleotide sequence data for accurate identification of functionally relevant genetic variants. Bioinformatics. 2023 Mar 1;39(3):btad101. doi: 10.1093/bioinformatics/btad101. PMID: 36825843; PMCID: PMC9994790.

Blanch-Asensio M, Dey S, Sankaran S. In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1. PLoS One. 2023 Feb 16;18(2):e0281625. doi: 10.1371/journal.pone.0281625. PMID: 36795741; PMCID: PMC9934402.

Bhusari S, Kim J, Polizzi K, Sankaran S, Del Campo A. Encapsulation of bacteria in bilayer Pluronic thin film hydrogels: A safe format for engineered living materials. Biomater Adv. 2023 Feb;145:213240. doi: 10.1016/j.bioadv.2022.213240. Epub 2022 Dec 20. PMID: 36577192

Seyfert CE, Porten C, Yuan B, Deckarm S, Panter F, Bader CD, Coetzee J, Deschner F, Tehrani KHME, Higgins PG, Seifert H, Marlovits TC, Herrmann J, Müller R. Darobactins Exhibiting Superior Antibiotic Activity by Cryo-EM Structure Guided Biosynthetic Engineering. Angew Chem Int Ed Engl. 2023 Jan 9;62(2):e202214094. doi: 10.1002/anie.202214094. Epub 2022 Dec 7. PMID: 36308277; PMCID: PMC10107326.

C. E. Seyfert, C. Porten and R. Müller*: Darobactin – ein neues Antibiotikum in Entwicklung BIOspektrum 2023, 05, 539.

Weiland F, Barton N, Kohlstedt M, Becker J, Wittmann C. Systems metabolic engineering upgrades Corynebacterium glutamicum to high-efficiency cis, cis-muconic acid production from lignin-based aromatics. Metab Eng. 2023 Jan;75:153-169. doi: 10.1016/j.ymben.2022.12.005. Epub 2022 Dec 20. PMID: 36563956

2022

Jungmann L, Hoffmann SL, Lang C, De Agazio R, Becker J, Kohlstedt M, Wittmann C. High-efficiency production of 5-hydroxyectoine using metabolically engineered Corynebacterium glutamicum. Microb Cell Fact. 2022 Dec 28;21(1):274. doi: 10.1186/s12934-022-02003-z. PMID: 36578077; PMCID: PMC9798599.

Mohr T, Zwick A, Hans MC, Bley IA, Braun FL, Khalmurzaev O, Matveev VB, Loertzer P, Pryalukhin A, Hartmann A, Geppert CI, Loertzer H, Wunderlich H, Naumann CM, Kalthoff H, Junker K, Smola S, Lohse S. The prominent role of the S100A8/S100A9-CD147 axis in the progression of penile cancer. Front Oncol. 2022 Oct 11;12:891511. doi: 10.3389/fonc.2022.891511. PMID: 36303837; PMCID: PMC9592847.

Rehner J, Schmartz GP, Groeger L, Dastbaz J, Ludwig N, Hannig M, Rupf S, Seitz B, Flockerzi E, Berger T, Reichert MC, Krawczyk M, Meese E, Herr C, Bals R, Becker SL, Keller A, Müller R; IMAGINE Consortium. Systematic Cross-biospecimen Evaluation of DNA Extraction Kits for Long- and Short-read Multi-metagenomic Sequencing Studies. Genomics Proteomics Bioinformatics. 2022 Apr;20(2):405-417. doi: 10.1016/j.gpb.2022.05.006. Epub 2022 Jun 6. PMID: 35680095; PMCID: PMC9684153.

Bhusari S, Sankaran S, Del Campo A. Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity. Adv Sci (Weinh). 2022 Jun;9(17):e2106026. doi: 10.1002/advs.202106026. Epub 2022 Apr 11. PMID: 35404519; PMCID: PMC9189655.

Tóth G, Berkó-Göttel B, Seitz B, Langenbucher A, Stachon T, Pluzsik MT, Nagy ZZ, Smola S, Szentmáry N. Herpes simplex virus PCR in 2230 explanted corneal buttons. Acta Ophthalmol. 2022 Feb;100(1):e77-e82. doi: 10.1111/aos.14872. Epub 2021 Jun 14. PMID: 34126653.

Isabelle Ariane Bley, Anabel Zwick, Muriel Charlotte Hans, Katrin Thieser, Viktoria Wagner, Nicole Ludwig, Oybek Khalmurzaev, Vsevolod Borisovich Matveev, Philine Loertzer, Alexey Pryalukhin, Arndt Hartmann, Carol-Immanuel Geppert, Hagen Loertzer, Heiko Wunderlich, Carsten Maik Naumann, Holger Kalthoff, Kerstin Junker, Sigrun Smola, Stefan Lohse, DKK1 inhibits canonical Wnt signaling in human papillomavirus-positive penile cancer cells, Translational Oncology, Volume 15, Issue 1, 2022.

2021

S. Pearson, J. Feng, A. del Campo Lighting the Path: Light Delivery Strategies to Activate Photoresponsive Biomaterials In Vivo Advanced Functional Materials, 2021, 31, (50), 2105989

Rodrigo-Navarro, A., Sankaran, S., Dalby, M.J. et al. Engineered living biomaterials. Nat Rev Mater 6, 1175–1190 (2021). https://doi.org/10.1038/s41578-021-00350-8

Groß S, Panter F, Pogorevc D, Seyfert CE, Deckarm S, Bader CD, Herrmann J, Müller R. Improved broad-spectrum antibiotics against Gram-negative pathogens via darobactin biosynthetic pathway engineering. Chem Sci. 2021 Aug 12;12(35):11882-11893. doi: 10.1039/d1sc02725e. PMID: 34659729; PMCID: PMC8442675.

Bhusari S, Sankaran S, Del Campo A. Regulating Bacterial Behavior within Hydrogels of Tunable Viscoelasticity. Adv Sci (Weinh). 2022 Jun;9(17):e2106026. doi: 10.1002/advs.202106026. Epub 2022 Apr 11. PMID: 35404519; PMCID: PMC9189655.

2020

Ahmed Y, Rebets Y, Estévez MR, Zapp J, Myronovskyi M, Luzhetskyy A. Engineering of Streptomyces lividans for heterologous expression of secondary metabolite gene clusters. Microb Cell Fact. 2020 Jan 9;19(1):5. doi: 10.1186/s12934-020-1277-8. PMID: 31918711; PMCID: PMC6950998.

S Dey, M Blanch Asensio, S Sankaran
“Neuartige genetische Werkzeuge”.
DE 10 2022 119 024.2

CE Seyfert, FT Panter, R Müller, S Deckarm, S Gross, D Scherzer, C Bader, J Herrmann
Novel darobactin derivatives
WO 2022/175443 A1

A del Campo Bécares, S. Sankaran, M Puertas Bartolomé, F Riedel
Lebendes Kompositmaterial für medizinische Produkte
DE 10 2023 131 356 A1

C Wittmann, A del Campo Bécares, M. Puertas Bartolomé, LL Teruel Enrico
Selbst-schmierende Kontaktlinsen
DE 10 2023 131 358 A1