Leading PI: Matthias Laschke (Saarland University)
The project aims to analyze the biocompatibility of shielded living therapeutics both in vitro and in vivo. In vitro assays will be established to investigate cytotoxicity and various properties of the materials after seeding with different cell types. To study in vivo the acute host tissue response to the materials, small samples will be implanted into mouse dorsal skinfold chambers and imaged over 2 weeks to assess inflammatory reactions and microvascular parameters. Additionally, materials will be implanted subcutaneously into mice for 2-3 months to study chronic host tissue response. The implants and surrounding tissue will be analyzed for vascularization, immune cell infiltration, and tissue incorporation. Later stages will include investigating the secretome, metabolomics, and proteomics patterns of the implanted materials.
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